The Gram stain is the most widely used staining procedure in the field of microbiology. It differentiates bacteria based on staining characteristics due to differences in cell wall compositions.
The Gram stain was first used in 1884 by Hans Christian Gram, a Danish bacteriologist. Gram was searching for a method that would allow visualization of cocci in tissue sections of lungs of those who had died of pneumonia. Gram was a modest man and in his initial publication, he remarked, “I have therefore published the method, although I am aware that as yet it is very defective and imperfect; but it is hoped that also in the hands of other investigators it will turn out to be useful.”
Gram staining is still known as one of the most important staining techniques used in microbiology to rapidly categorize bacteria under a microscope. In this video below, Hardy Diagnostics honors the Gram stain method by explaining how to perform a perfect Gram stain. This training video will prove to be useful for both new and experienced technologists.
By Megan Roesner
Clinical Marketing Coordinator